Evaluación de una solución inmovilizadora para criopreservación del semen de Colossoma macropomum, “Gamitana”

International audienceThe objective was the cryopreservation of semen, which enable a gene bank, for which a solution was sought inactivating semen collection for Colossoma macropomum "tambaqui" to obtain sperm with good motility after activation thawing in liquid nitrogen. The collection inactivating solution was prepared with semen from players held at the Institute of Amazonian Research (IIAP)-induced without inducing Conceptal ® and maintained at the station of New Horizon National Fisheries Development Fund (FONDEPES). Semen was collected in solutions of 9 % and 10 % NaCl, adding 2 g/L, 4 g/L and 8 g/L NaHCO3. As inactivating solution was tested 3 concentrations of sucrose (300 mM, 400 mM and 500 mM) alone or with 1.5 g/L, 1 g/L and 0.5 g/L NaCl. The treatment of 400 mM sucrose gave the best result, with 80% motility and 40 seconds long. Motility was also evaluated after one hour storage at room temperature with 60% motility after activation and 20 seconds long. This work will develop a protocol for cryopreservation of semen frozen batches, with sufficient time to prepare the straws, frozen in liquid nitrogen and optimize the management of players